Allergy methods and protocols

Meinir G. Jones, Penny Lympany

In this book leaders in the field provide assistance for researchers to gain insight into the molecular mechanisms involved in allergy by featuring an array of protocols. These cover a range of disciplines including allergy, immunology, cell biology and histology and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, T regulatory cells. Techniques discussed include; B and T cell epitope mapping, characterization of allergens, conjugation of haptens, preparation of monoclonal antibodies, collection and sampling of airborne allergens, IgG antibodies and facilitated antigen blocking assays, identification and purification of mast cells and in situ hybridisation.